BREEDING
INSTRUCTIONS
FOR FROZEN
SEMEN PROCESSED BY ALLENDALE FARMS, LLC.
Step 1 – The
Mare
The mare must be monitored throughout her heat cycle for follicle
growth. When ovulation is imminent (38- 44mm), mare should
be checked every 6-12 hours to detect ovulation. When ovulation
has been confirmed, the semen straws should be thawed
, and inseminated . ( Donkey and Zebra mares ovulate between
35mm-40mm.)
DO NOT
breed prior to ovulation.
Step 2 –
Thawing the Semen Straws
Confirm the cane ID and the straw color on your Frozen Semen Work
Sheet. Also check the number of straws per dose. Prior to
retrieving the straws from the liquid nitrogen tank, you will need
to prepare two (2) water baths. The 1st bath should be
45° C and the 2nd
bath should be 37° C. Thaw
one (1) straw at a time. The straw should be removed from the
liquid nitrogen tank and immediately put into the 1st
water bath for 10 seconds. Hold the straw under the water (straw
will try to float). Immediately place the straw in the 2nd
water bath for 30 seconds minimum. NEVER let any straws be
exposed to outside air temperatures (raised above the frost line in
the liquid nitrogen tank) for over 4 seconds unless it is being
thawed. Four (4) seconds should give you ample time to retrieve the
straw to be thawed. After thawing, shake the air bubble to the
sealed end. DO NOT cut the wick end of the straw.
Step 3
Artificial Insemination of the Mare
IMMEDIATELY after thawing the straws, artificially
inseminate (AI) the mare. Procedure # 2 may be more
practical.
Procedure 1:
Deep uterine insemination: Use ˝ ml straw AI pipette. (flexible
models are available thru
Minitube of America - 800-646-4882.,stylet #17209/1075,
pipette #17209/1275) It is important the tip of the pipette is in
the uterine horn toward the ruptured follicle.
Procedure 2:
Use 8-10 ml Next Generation Basic Formula Extender, or equivalent
(no antibiotics). Empty all thawed straws into the extender. Mix
by inversion. DO NOT SHAKE. Use a standard equine AI
pipette and a ALL PLASTIC syringe.
Flush the AI tube with 4 ml of plain extender to wash all semen
through the pipette.
IMPORTANT:
EXTENDER MUST BE THE EXACT TEMPERATURE AS THE STRAWS WHEN MIXING.
Suggestions
Do not use abnormal mares.
AI all semen into the uterine horn toward the ruptured follicle.
(flexible pipette)
It may be difficult to check motility using AI procedure 1. (Apply
enough semen on slide for cover slip to float across entire
sample.)
Do not use soap or iodine to clean mare. Use water only.
Monitor the liquid nitrogen level in your tank weekly. In the
unlikely event your tank loses all of it’s liquid nitrogen, discard
every straw in the tank. Keep your tank in plain view. Ice or frost
should not form on outside of tank. If you suspect a tank
failure, move semen to another tank immediately. A thawed straw
cannot be refrozen.
Keep your liquid nitrogen tank, work sheets and these instructions
in a safe place.
Do not allow liquid nitrogen tanks to come in direct contact with
concrete, asphalt or another tank.
These instructions are for information purposes only, and should not
be used in some States.
Tennessee code annotated 63-12-103 / 9.a. (1) Kentucky
revised statutes 321.181 5.a.
If you have any questions contact Allendale Farms LLC at
706-476-5415.
Allendale Farms LLC operates in
accordance with HB 347, Title 43 of the Official Code of Georgia
Annotated. Ref: 43-50-44 Collection, evaluation and storage of
semen. Revised 4/17/03
Revised June 15, 2005
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